A.N.T. Farm revolves around Chyna Parks an 11-year-old musical prodigy, who has just become the newest student in the A.N.T. program at Webster High School in San Francisco, California. On her first day, she meets Gibson, the goofy counselor, tutor, and therapist of the A.N.T. Farm, as well as Olive Doyle, , a girl with an eidetic memory, and Fletcher Quimby,an artistic genius (but is otherwise quite dim) who falls deeply in love with Chyna when they first meet. After quickly becoming friends, they proceed to go on multiple adventures, using their talents to their advantage.
- China Anne McClain as Chyna Parks
- Sierra McCormick as Olive Doyle
- Jake Short as Fletcher Quimby
- Stefanie Scott as Lexi Reed
- Carlon Jeffery as Cameron Parks, Chyna's older brother (Seasons 1-2)
- Aedin Mincks as Angus Chestnut (Season 3)